Figure 6
RNAi knockdown of TmIMD enhanced the susceptibility of T. molitor larvae to E. coli and C. albicans infection. (A) T. molitor larvae (10–12th instar) were injected with dsTmIMD (2 µg per insect) and transcript silencing was measured by qPCR. The EGFP dsRNA-treated larvae served as a negative control. Data represent means ± standard error of three independent biological replicates. E. coli (B), S. aureus (C), and C. albicans (D) were inoculated at 2-days post RNAi, and larval survival was recorded daily for 10 days in each treatment group. The experiment was repeated three times with similar results. Statistical analysis of survival analysis was carried out based on Kaplan-Meier plots (log-rank chi-square test; *p < 0.05).
