Figure 2
Blockade of LTCC suppressed DOX-induced phosphorylation of CaMKII and NF-κB and increases in cleaved caspase 3 in cardiomyocytes. (a) Representative immunoblots of CaMKII, phosphorylated CaMKII, NF-κB, phosphorylated NF-κB, cleaved caspase 3, and GAPDH in NRVMs treated with or without nifedipine (Nif, 1 μM) in the presence or absence of DOX (10 μM) for 24 hour (n = 5). The experiment was conducted 3 times. (b–d) Quantitative analysis of phosphorylated CaMKII, phosphorylated NF-κB, and cleaved caspase 3 in each group (n = 5). (e) Representative immunoblots of CaMKII, phosphorylated CaMKII, NF-κB, phosphorylated NF-κB, cleaved caspase 3, and GAPDH in NRVMs treated with indicated small interfering RNA (siRNA) in the presence or absence of DOX (10 μM) for 24 hour (n = 5). (f–h) Quantitative analysis of phosphorylated CaMKII, phosphorylated NF-κB, and cleaved caspase 3 in each group (n = 5). The experiment was conducted 3 times. *P < 0.05, **P < 0.01: post-hoc Tukey’s comparison test.
