Figure 1

Wood Smoke and Cell Viability. (A) MTT and (B) trypan blue exclusion were used to assess the effects of WS on A549 cell viability. Staurosporine was used as a positive control for reduced viability. MTT *P < 0.001 vs. 24 h 5% WS, 10% WS; ^†P < 0.001 vs. 24 h 10% WS, ^‡P < 0.001 vs. 24 h 5% WS, ^ΨP < 0.001 vs. 24 h PBS, ^&P < 0.01 vs. 24 h Stauro, ^ΥP < 0.001 vs. 24 h Stauro, ^$P < 0.001 vs. 48 h PBS, 5% WS, 10% WS; n = 3). Trypan blue exclusion (*P < 0.05 vs. 24 h Unt and 24 h 5% WS; ^†P < 0.001 vs. 24 h Unt; ^‡P < 0.001 vs. 24 h 5% and 10% WS and 48 h Unt; ^ΦP < 0.05 vs. 48 h 5% and 10% WS; n = 3). Statistical differences were determined using a two-way ANOVA followed by a Tukey’s post-hoc test. Data presented as mean ± se.