Figure 6
Activation of MAPK Signaling following Wood Smoke Exposure. (A) WS exposure resulted in activation of a p44/42 dependent MAPK signaling pathway. After 24 hours of WS exposure there was approximately a 50% increase in p44/42 phosphorylation although this was not significant. After 48 hours, there was a significant increase in p44/42 phosphorylation as compared to untreated cells after both 24 and 48 hours (*P < 0.05 vs. 24 h Unt; †P < 0.05 vs. 48 h Unt; n = 4). (B) There was no evidence of p38 phosphorylation after 24 or 48 hours of exposure. EGF (10 ng/mL) stimulated cells were used as a positive control. BSA treated cells were used as a control for EGF stimulation. Images were cropped to show a representative image of each treatment and time point. All images were derived from the same blot following stripping and re-probing. Full blots are provided in Supplementary Figs S1, S2 and S3. Statistical differences were determined using a two-way ANOVA followed by a Tukey’s post-hoc test. Data presented as mean ± se.
