Figure 3
From: Dapagliflozin rescues endoplasmic reticulum stress-mediated cell death
Dapagliflozin regulates unfolded protein response through the eIF2α pathway in HK-2 cells. (a) HK2 cells were treated with 2 μM dapagliflozin for 36 h with varying concentrations of D-galactose. Immunoblotting was performed with the indicated antibodies. (b) HK2 cells were transfected by siRNA for elf2α and incubated with 2 μM dapagliflozin for 36 h. Immunoblotting was performed with the indicated antibodies. (c,d) HK2 cells were transfected by siRNA for elf2α and incubated with 2 μM dapagliflozin for 36 h. The mRNA expression levels of ATF4 and CHOP were determined by qRT-PCR (n = 5 independent experiments).
