Figure 4
From: Dapagliflozin rescues endoplasmic reticulum stress-mediated cell death
Dapagliflozin rescued C2 ceramide-induced ER stress in HK2 cells. (a) HK2 cells were treated with 2 μM dapagliflozin for 36 h with or without 7.5 ng/mL C2 ceramide, and immunoblotting was performed with the indicated antibodies (b,c). Signal quantification of the expression levels of GLUT2 (b) and SGLT2 (c) normalised with tubulin-α levels. (d) Signal quantification of the expression levels of S51 phosphorylation of elf2α normalised with total elf2α levels. (e) HK2 cells were incubated with 2 μM dapagliflozin for 36 h, and the mRNA expression levels of ATF4 were determined by qRT-PCR (n = 5 independent experiments).
