Figure 6

Effect of dapagliflozin on ER stress-induced cell death in vivo. (a) Body weights of db/db mice administered oral dapagliflozin every day for 1 week. (b) Blood glucose levels of db/db mice administered dapagliflozin. (c) SGLT2 mRNA levels in the kidney of db/db mice were determined by qRT-PCR (n = 5 independent experiments). (d,g) The kidneys of db/db mice were utilised for protein expression analysis. These immunoblots are representative of n = 5 experiments performed independently. (e) Signal quantification of the expression levels of S⁵¹ phosphorylation of elf2α normalised with total elf2α. (f) Expression levels of ATF4 mRNAs were determined by qRT-PCR (n = 5 independent experiments). (h,i) Signal quantification of the expression levels of ATF6 normalised with tubulin-α (h) and the expression levels of S⁷²⁴ phosphorylation of IRE1α normalised with total IRE1α (i).