Figure 10

Autophagy and mitophagy are increased during cardiac surgery in a hypothermic setting. WB analyses were performed on LV apical tissue obtained during LVAD procedure (n = 6 and n = 7 in the warm and cold group respectively). (A,B) WB analysis on whole lysate and mitochondrial fraction respectively. Protein quantification (C–E) of parkin, phospho-S6/S6 ratio and LC3-II in the whole lysate, (F,G) LC3-II and NDP52 in the mitochondrial fraction respectively. All proteins were normalized to ponceau. The data represents the mean ± SEM with *p < 0.05, comparing cold (black) vs. warm (white). We normalized each protein to the entire corresponding lane on the ponceau-stained membrane, representing the total protein amount. Due to space limitation, the blots are cropped from various gels, please see the supplemental data for full-length gels/blots.