Table 1 Overview of the used commercial antibodies and other reagents.
Name | Conjugate | Clone | Firm | Application |
|---|---|---|---|---|
Mouse anti CD32 | Biotin | AT10 | Bio-Rad | F |
Mouse anti-CD32 | n.a. | AT10 | Genetex | C |
Mouse anti-CD23 | PE | EBVCS-5 | BD Biosciences | F, C |
Mouse anti-CD23 | n.a. | BU38 | Ancell Corporation | C |
Mouse anti-CD209 | PE | MR-1 | Bio-Rad | F, C |
Mouse anti-DC-SIGN | n.a. | MR-1 | Bio-Rad | C |
Human IgE | APC* | n.a. | Abcam | F |
ICAM-3 His-tagged | n.a. | n.a. | Sino Biological | F |
Mouse anti-His tag | FITC | 6G2A9 | Genscript | F |
Mouse IgG1κ isotype | PE | 203 | Sanquin | F, C |
Mouse IgG1κ isotype | n.a. | 203 | Sanquin | C |
Goat anti-human IgG1 F(ab’)2 | PE | n.a. | Southern Biotech | F |
Streptavidin | APC | n.a. | Biolegend | F, E |
Streptavidin | HRP | n.a. | Sanquin | E |
Mouse anti-human IgG | n.a. | MH16-1 | Sanquin | E |
Mouse anti-human IgG | HRP | MH16-1 | Sanquin | E |
Sambucus nigra agglutinin | Biotin | n.a. | Elderberry Bark lectin, Vector Labs | E |
