Figure 6

Study time-line. Following initial weight and wellbeing assessment, 7 piglets were randomly assigned to immediate euthanasia (Naïve). Following baseline recordings and tracheostomy, remaining piglets were randomized to receive (i) Saline bolus and infusion alone (Sham, n = 3), (ii) LPS bolus and infusion alone (LPS, n = 5), (iv) hypoxia 4 h after saline bolus (Hypoxia, n = 6) and (v) hypoxia 4 h after LPS bolus (LPS + Hypoxia, n = 5). Blood samples were collected at baseline, 4 h after bolus, end of hypoxia (time 0), and at 3, 12, 24 and 48 h after time 0. Piglets were maintained under meticulous intensive care for 48 h following hypoxia, prior to euthanasia and histological specimen collection. EEG was acquired continuously throughout.