Figure 6
Fast SRH using the FM-SRS modality. (a) FM-SRS (SRSnuclei = \({{\rm{SRS}}}_{2930{{\rm{cm}}}^{-1}}\) − \({{\rm{SRS}}}_{2845{{\rm{cm}}}^{-1}}\)): nuclei distribution, (b) CARS, and (c) TPEF: cell body distribution and (d) SHG: collagen distribution are acquired simultaneously. Combining (a–d), a composite image can be built: FMSRS (blue), TPEF (green) and SHG (magenta). Applying the LUTs described in the Materials and Methods section a SRH image can be built (f-g-h). (f) SRH from raw data, (g) image smoothed and color adjusted from (f). (h) SRH on a large millimeter-scale region on healthy (i.e. non-cancerous) human colon tissue. Acquisition time 25 minutes. Scale bar 100 μm.
