Figure 5 | Scientific Reports

Figure 5

From: A 4-cyano-3-methylisoquinoline inhibitor of Plasmodium falciparum growth targets the sodium efflux pump PfATP4

Figure 5

Reduction of RhopH2 expression reduces the lysis of infected erythrocytes by sorbitol entry via new permeability pathways (NPPs). (A) Diagram of the rhopH2 locus tagged with haglmS. (B) Trophozoite stage Hyp1-Nluc and RhopH2-HAglmS infected erythrocytes (IE) were treated with the concentrations of glucosamine (GlcN) indicated for 48 h days to reduce the expression of RhopH2-HA in RhopH2-HAglmS parasites. Densitometry of western blot bands from three independent experiments are shown with each point indicating mean and SD. Full size blots can be viewed in Fig. S9. (C) The reduction of RhopH2-HA expression reduced the susceptibility of RhopH2-HAglmS infected cells to sorbitol induced lysis relative to Hyp1-Nluc 3D7 infected control cells. The lysis of IE was quantified by measuring the bioluminescence of Hyp1-Nluc released from the infected IE.

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