Figure 8
The NPP-blocking compound furosemide protects parasite infected erythrocytes from lysis induced by PfATP4 inhibitors cipargamin and MB14. Erythrocytes infected with RhopH2-HAglmS trophozoites expressing exported nanoluciferase were treated with either 20 µM MB14 or 20 nM cipargamin for 20 min then resuspended in medium in either the presence or absence of 100 µM furosemide. Compound dependent lysis is the percentage of relative light units (RLU) produced by nanoluciferase released into the media from lysed infected RBCs divided by the total RLU of the whole culture (media and cells). One representative experiment of the three performed (each of which contained three technical repeats) is shown here. Error bars indicate SD, ****p < 0.0001, unpaired T-test.
