Figure 3

Procedure of multiple drug dispensing, cell loading and evaluation of drug toxicity by the nanodroplet cell processing platform. The left top panel shows the fabricated ParaStamp and the corresponding 9 × 9 ParaStamp chip on a 22 × 22 mm2 cover glass, respectively. The left bottom panel shows the representative micrograph of two circular wax-patterned wells. The diameter (D) of the wells are 1151 ± 39 μm (coefficient of variation, CV = 3.4%, n = 32). The volume of each droplet dispensed is 200 nl. Scale bar, 500 μm.