Table 1 Funnel Strategy −2,532 compounds screened.

From: Identifying chemopreventive agents for obesity-associated cancers using an efficient, 3D high-throughput transformation assay

Hit Parameter

Identified Hits

Hit Percentage

Transformation HTS −>50% inhibition

178

7.0

Cytotoxicity −<25% decrease in HEK293 cell viability)

105

4.2

Promiscuity - active in <20% assays listed in Pubchem

58

2.3

Commercially available compounds - DTP/CC

33

1.3

Fresh powder confirmation −>40% inhibition

7

0.27

  1. Abbreviations: HEK293, human embryonic kidney; DTP, Developmental Therapeutics Program; CC, Cayman Chemical.
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