Figure 2

Relationship of cTfh cells and their subsets with HCV neutralizing antibody responses. (A–D) Correlations of the neutralization titer (genotype 1a is shown as a representative) with cTfh cells (A), CXCR3⁺ cTfh cells (B), CXCR3⁻ cTfh cells (C), and the ratio of CXCR3⁺/CXCR3⁻ Tfh cells (D) in CD4⁺ T cells in HCV infection (n = 38). (E-F) Correlations of the neutralization breadth with cTfh cells (E), CXCR3⁺ cTfh cells (F), CXCR3⁻ cTfh cells (G), and the ratio of CXCR3⁺/CXCR3⁻ Tfh cells (H) in CD4⁺ T cells in HCV infection (n = 38). Neutralization titers were calculated by combining fourfold dilutions of serum (dilution started from 1:100) with HCVpps. The neutralizing breadth was indicated by the number of HCVpps neutralized >50%; positive neutralization was categorized as a reduction in infectivity by 50% in this HCVpp system. Spearman R and P values are indicated.