Table 2 Diffusion and detection of example root exudate metabolites through nanoporous membrane.

From: Label-free time- and space-resolved exometabolite sampling of growing plant roots through nanoporous interfaces

Metabolite

%C/C0 Fraction diffuseda

LLOD (nM)

LLOD (µM)

LLOD (nM)

Standard

Culture micro

environment

Metabolite

sampling layerb

Valine

0.19 ± 0.025

1.3

1.0

1.6

Alanine

0.16 ± 0.015

1.5

1.0

1.6

Isoleucine

0.14 ± 0.023

1.3

1.6

1.5

Phenylalanine

0.12 ± 0.013

11

10

11

Sucrose

0.10 ± 0.0047

27

48

42

Malic acid

0.10 ± 0.0018

1.4 × 102

2.1 × 102

1.8 × 102

  1. a%C/C0 Fraction diffused was determined from amount of materials present in the 20 µL of sample microenvironment and 20 µL metabolite sampling layer after 8 h of diffusion through 50 × 50 µm window.
  2. bThe detection limit of metabolite sampling layer corresponds to that of sample environment.
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