Table 3 Calculated Kd values for ligands binding to WT HtxB and D206N/D206A HtxB mutants at pH 7.4 or pH 5 (NBD = no binding detected up to 100 mM ligand).

From: Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand

Protein

Hypophosphite (μM)

Phosphite (μM)

pH 7.4

pH 5

pH 7.4

pH 5

WT

0.65 ± 0.13

1.46 ± 0.16

NBD

1.74 × 103 ± 0.221 × 103

D206A

3.24 × 103 ± 0.93 × 103

7.76 × 103 ± 5.07 × 103

NBD

NBD

D206N

NBD

NBD

NBD

NBD

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