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Figure 1

From: Loss of Cubilin, the intrinsic factor-vitamin B12 receptor, impairs visceral endoderm endocytosis and endodermal patterning in the mouse

Figure 1

Expression of Cubn mRNA and protein prior to gastrulation. (A,C and E) Whole mount in situ hybridization of Cubn mRNA (A) at embryonic day 3.5 (E3.5) showing Cubn expression at the level of the primitive endoderm (PrE). (C) At the late blastocyst stage E4.5, strong Cubn mRNA expression is found in the PrE. (E) At the early postimplantation stage E5.25, Cubn expression is enriched in the extraembryonic visceral endoderm (exVE). (B,D and F) Whole mount immunofluorescence detects Cubilin (in green) in the cytoplasm of the PrE (B) and subapical region of the PrE cells. (D) Phalloidin is in red; nuclei colored by Hoechst are shown in blue. (F) At E5.25, Cubilin protein is detected both in the extraembryonic and embryonic subapical region of the VE cells. ant: anterior, ICM: inner cell mass, emVE: embryonic visceral endoderm, Epi: epiblast, post: posterior, Te: trophectoderm. Scale Bars: 20 μm in (A,B) 25 μm in (C,D) 40 μm in (E,F).

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