Figure 8
Altered expression of endocytic markers in the exVE of Cubn mutants. (A) Cubilin (red) and Amn (green) colocalize at the plasma membrane. A faint Amn signal is also observed in the large subapical vesicles (arrow). (B) Lrp2 is mainly localized at the plasma membrane. (C) LAMP1 marks lysosomes that display large vacuolar morphologies in the exVE cells. The faint vesicular Amn immunoreactivity is found in LAMP1-positives vesicles (arrow). (D) EEA1 is a marker of endosomal processing and decorates small disc-shaped structures underneath the apical plasma membrane (arrow) that are distinct from LAMP1 vesicles. (E) In Cubn0/0 embryos, Amn displays an abnormal dispersion in the cytoplasm whereas (F) Lrp2 staining remains apical. (G) In Cubn0/0 embryos, LAMP1- or Amn-positive large vacuoles are absent. LAMP1- positive smaller and denser vacuoles are observed underneath the plasma membrane (arrowheads). (H) In Cubn0/0 embryos, both EEA1 and LAMP1 vesicles are localized underneath the apical plasma membrane. EEA1 decorates small dense vesicles (arrows) distinct from the small LAMP1-positive vesicles (arrowheads). Scale bars: 15 μm.
