Figure 2
(a–d) SHG images of the in vivo corneal stroma (apex region) of two different subjects (#1 (a, c); #2 (b,d) and two different depth locations: (a,b) anterior stroma ~50-µm depth; (c,d) posterior stroma ~350-µm depth. (e,f) Comparison of SHG images of the corneal stroma recorded in in vivo and ex vivo conditions, where the spatial pattern could be clearly visualized. The arrows indicate the two POs. Scale bar: 50 µm. The image corresponding to the ex vivo sample was recorded as explained in ref.21. (g,h) PO histograms showing the two orientations of the fibers (peaks). Each in vivo SHG image corresponds to an individual frame (image averaging was not used). Scale bar: 50 µm.
