Figure 5
(a) Schematic diagram of the compact 2P microscope. S, shutter; BE, beam expander; NDF, neutral density filter; AP, aperture; L1 and L2, achromatic doublets; DM, dichroic mirror; OB, microscope objective; SF, spectral filter; PMT, photomultiplier tube; DAQ, data acquisition card; PC, computer. (b) Picture showing a top view of the actual instrument. The main elements are highlighted. (c) Customized user’s interface used for eye centration and image recording. The operator can choose the dimensions of the scanning area, the image size (in pixels) and the exposure time. For optical sectioning, the user must also set the number of planes and the axial distance between correlative planes.
