Figure 1 | Scientific Reports

Figure 1

From: Depletion of Ras Suppressor-1 (RSU-1) promotes cell invasion of breast cancer cells through a compensatory upregulation of a truncated isoform

Figure 1

Comparison of the gene sequences of RSU-1L and RSU-1-X1 and effect of RSU-1L depletion from MDA-MB-231-LM2 cells on the expression of the truncated RSU-1-X1 isoform. (a) Comparison of the gene sequences of RSU-1L and RSU-1-X1. Trapezoids drawn between the bars indicate the portions of the sequences that align to each other, while the white area corresponds to the missing sequence in RSU-1X1 isoform (nucleotides 598–731, total missing piece of 133 bp). (b) Representative western blot using total cell lysates from MCF-7 and MDA-MB-231-LM2 cells stably expressing scrambled control shRNA (SshRNA) or shRNA against RSU-1L (RSU-1L shRNA). Two RSU-1 isoforms are identified while β-actin was used as loading control. (c–e) Real Time PCR-mediated analysis of mRNA expression using primers that recognize both RSU-1 isoforms (c), only RSU-1L (d) or only RSU-1-X1 (e) isoform. B-actin was used as the housekeeping gene and S-shRNA-treated cells served as calibrator for the ΔΔCt method. Experiments were performed in triplicates and four (4) independent experiments were conducted. Asterisks indicate statistically significant changes (*p-value < 0.05, **p value < 0.01, ***p value < 0.001). Full-length blots are presented in Supplementary Fig. 1.

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