Figure 6 | Scientific Reports

Figure 6

From: Depletion of Ras Suppressor-1 (RSU-1) promotes cell invasion of breast cancer cells through a compensatory upregulation of a truncated isoform

Figure 6

Effect of silencing of one or both of the RSU-1 isoforms in tumor spheroids invasion. Tumor spheroid invasion assay performed in spheroids from MDA-MB-231-LM2 cells transfected with Non Specific control (NSC) siRNA and control shRNA at zero and 18 h post embedding into collagen gels (a,b), control shRNA and RSU-1-X1 siRNA (e,f), RSU-1LshRNA and control NSC siRNA (c,d) and both RSU-1X1 siRNA and RSU-1L-shRNA (g,h). (i) Quantification of the cell invasion results from two independent experiments with at least 8 spheroids per condition. (j) Quantification of the mRNA expression of urokinase Plasminogen Activator (uPA) under the aforementioned conditions by Real Time PCR. Asterisks denote statistical significant differences (*p value < 0.05, **p value < 0.01, ***p value < 0.001).

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