Figure 1
From: Imaging axon regeneration within synthetic nerve conduits
Testing the optical properties of different types of conduits after clearing. (A) A cotton thread wrapped around different types of conduits was subjected to a modified CUBIC clearing protocol and imaged by confocal microscopy. Two different types of collagen conduits were used in this conduit screen, AxoGuard® and NeuraGen®, as well as a PCL conduit, NEUROLAC®, and a chitosan conduit. The NEUROLAC® conduit was optically transparent and served as a positive control. (B) The conduits were soaked in an NaBH4 solution overnight prior to clearing, in order to quench autofluorescence. Chitosan was sufficiently responsive to NaBH4 but collagen was not. Scale bar length is 100 µm.
