Figure 3
From: Imaging axon regeneration within synthetic nerve conduits
Quantification of axonal growth within conduits. (A) Schematic of the nerve-conduit assembly, as used in the sciatic nerve transection paradigm (dimensions in µm). The entire conduit was approximately 4 mm in length. The segment in the proximal stump between −1000 µm and −500 µm was used for normalizing the number of intersections per increment to the mean number of GFP-positive fibers in the intact nerve. Individual nerves were aligned by means of the lesion plane, the position of which was denoted by “0”. (B) Read-out from a single experiment. The number of intersections was normalized to the mean value of intersections in the “−1000 to −500” segment and plotted against the corresponding position on the nerve-conduit axis. The lesion plane is indicated with a red bar.
