Figure 8 | Scientific Reports

Figure 8

From: Construction, Expression and Evaluation of Recombinant VP2 Protein for serotype-independent Detection of FMDV Seropositive Animals in Egypt

Figure 8

(A) Standardization of rVP2 protein-based ELISA by checkerboard titration between rVP2 protein and serum antibodies from naïve calves vaccinated with the local inactivated polyvalent O, A, and SAT 2 oil vaccine, in ELISA. Twofold serum dilution starting with a dilution of 1:8, and serial dilution of rVP2 ranged from 300 to 50 ng/well. The negative control reaction is PBS. (B) Applicability of rVP2-based ELISA showing the detection of FMDV antibodies in sera of clinical cases collected during field outbreaks (155 out of 172), and in naïve calves vaccinated with the local inactivated polyvalent O, A, and SAT 2 oil vaccine (328 out of 328), while FMDV free commercial sera used as controls did not result in any detectable signals.

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