Figure 5

Co-cultures of WT variant D (squares) and LasR-null mutants of variant D (circles) in 0.75% adenosine broth. Initial frequency of the LasR mutant was 1% (filled symbols) or 10% (open symbols). (a) Cell density, determined as CFU (see Methods). (b) LasR mutant frequency, calculated from data in panel a. (c) Growth curves of adenosine-evolved variant D (filled squares) and a LasR mutant of variant D (open circles) in 0.75% adenosine. OD₆₀₀ was measured in a 96 well plate, using a microplate reader. All data are means of three replicates and the error bars represent the SEM of the data.