Table 1 Bacterial strains and plasmids used in this study.
From: Dynamics of cheater invasion in a cooperating population of Pseudomonas aeruginosa
Strain or plasmid | Relevant properties | Reference or origin |
|---|---|---|
P. aeruginosa | ||
PAO1 | Wild-type | |
PAO1-lasR | PAO1 containing an unmarked, in-frame lasR deletion | |
PAO1-psdR | PAO1 containing an unmarked, in-frame psdR deletion | |
PAO1-psdRlasR | PAO1-psdR containing an unmarked, in-frame lasR deletion | This work |
PAO1-variant D | Adenosine growth variant containing gene amplification of the sequence 162685–175070 | |
PAO1-variant D-lasR | PAO1-variant D containing an unmarked, in-frame lasR deletion | This work |
PAO1-mcherry | chromosomal insertion: attTn7:: Ptac-mCherry | This work |
PAO1-lasR-GFP | Gmr; chromosomal insertion: attTn7:: Ptac-GFP | This work |
PAO1-psdR-GFP | chromosomal insertion: attTn7:: Ptac-GFP | This work |
PAO1-psdRlasR-mcherry | Gmr; chromosomal insertion: attTn7:: Ptac-mCherry | This work |
PAO1-variant D-mcherry | chromosomal insertion: attTn7:: Ptac-mCherry | This work |
PAO1-variant D-lasR-GFP | Gmr; chromosomal insertion: attTn7:: Ptac-GFP | This work |
Escherichia coil | ||
S17-1 | recA pro hsdR RP4-2Tc::Mu-Km::Tn7 | |
NEB5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NEB |
Plasmids | ||
pUC18-mini-Tn7-Gm-GFP | Used for the integration of gfp at the att site | |
pUC18-mini-Tn7-Gm-mCherry | Derived from pUC18-mini-Tn7T-Gm31; used for the integration of mCherry at the att site | |
pPEXG2-lasR | pEXG2 containing sequences for lasR knockout | |
pFLP2 | flp recombinase gene for excising a resistance marker from the fluorescent protein cassettes delivered in the pUC18-mini-Tn7T-Gm plasmids | |
