Figure 1
From: LC3-positive structures are prominent in autophagy-deficient cells
(a) Representative immunofluorescence images showing LC3 staining in ATG9 control and knockout cells. (b,c) Quantification of the LC3 puncta average size and average number from the images in (a) performed using ImageJ quantification tool (**p < 0.01, ***p < 0.001). Quantification performed from 3 experiments with >25 cells quantified for each condition. Error bars represent standard deviation (SD). (d) Representative images showing the rescue of the phenotype observed in ATG9-knockout cells using ATG9A-GFP construct. (e,f) Quantification of the LC3 puncta average size and average number from the images in (d) performed using ImageJ quantification tool (**p < 0.01, ***p < 0.001). Quantification performed from 3 experiments with >25 cells quantified for each condition. Error bars represent standard deviation (SD). (g) Representative immunofluorescence images showing LC3 staining in ATG16L1 control and knockout cells. ATG16 control and knockout cells were fixed with methanol and labelled for endogenous LC3. (h) Quantification of the average number of LC3 puncta as observed in the images shown in panel (g) performed using ImageJ quantification tool (**p < 0.01, ***p < 0.001). Quantification performed from 2 experiments with >25 cells quantified for each condition. Error bars represent standard deviation (SD). Scale bars represent a distance of 10 µm.
