Figure 4
From: LC3-positive structures are prominent in autophagy-deficient cells
(a) Representative immunofluorescence images showing the co-localization of LC3-positive structures in autophagy-deficient ATG9 and ATG16L1 knockout cells with p62 using co-localisation profile generation. The co-localisation pixels for the image were identified, and a profile was generated using an unsupervised ImageJ plugin algorithm called colocalization, which was developed by Pierre Bourdoncle (Institut Jacques Monod, Service Imagerie, Paris; 2003–2004). (b) Representative immunofluorescence images showing the co-localization of p62 structures in autophagy-deficient ATG9 and ATG16L1 knockout cells with ubiquitin using co-localisation profile generation. The co-localisation pixels for the image were identified, and a profile was generated using an unsupervised ImageJ plugin algorithm called colocalization, which was developed by Pierre Bourdoncle (Institut Jacques Monod, Service Imagerie, Paris; 2003–2004). Scale bar represents a distance of 10 µm.
