Figure 5 | Scientific Reports

Figure 5

From: Indomethacin reduces rates of aortic dissection and rupture of the abdominal aorta by inhibiting monocyte/macrophage accumulation in a murine model

Figure 5

Indomethacin attenuates transendothelial migration of monocytes. (a) Scatter dot plots showing the means ± SD of Ccl2, Ccl7, and Ccl12 mRNA expressions in ΔCt in the aortas from sham (n = 12), BAPN and Ang II infused (n = 18), or BAPN and Ang II infused with indomethacin administered (n = 11) mice. One-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 (b) Scatter dot plots showing the means ± SD of the fold change of transendothelial migration activity of splenic monocytes stimulated with 100 μM BAPN and 1 ng/ml Ang II in the presence or absence of 10−6 M indomethacin from that of unstimulated monocytes. *P < 0.05 n = 6 per group. Four outliers were identified and removed by the ROUT function on GraphPad Prism prior to Mann-Whitney U Test. (c) Scatter dot plots showing the means ± SD of PECAM-1 expression on peripheral blood monocytes measured as mean fluorescence intensity. Peripheral blood was collected after 4 hours of BAPN/Ang II administration and processed for flow cytometry. Vehicle group: n = 5. Indomethacin group: n = 5. Mann-Whitney U Test. *P < 0.05 (d) Scatter dot plots showing the means ± SD of PECAM-1 expression on aortic endothelial cells measured as mean fluorescent intensity. Cells stained with tomato lectin were measured for fluorescence intensity for PECAM-1 (n = 5). Mann-Whitney U Test.

Back to article page