Figure 3 | Scientific Reports

Figure 3

From: Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression

Figure 3

Adipose tissue size regulators EPAC1 and EPAC2 are express in EAT and EPAC2 directly correlates with IL1RL1 expression. EPAC proteins, the main effectors of cAMP as controllers of adipose tissue size and metabolism, are expressed by EAT from CVD patients. (a) EPAC1 isoform is significantly overexpressed compared to the EPAC2 isoform in EAT cells, as shown by microarray results (p < 0.0001) but the total protein production, analyzed by Western blot and quantified normalizing the data using stain-free gels, indicated higher expression of EPAC2 protein than EPAC1, although the difference was not significant. (b) Correlation analysis between the expression of EPAC1 and EPAC2 adipose tissue size controllers and the ST2 cardiac stretch mediator in dysfunctional EAT biopsies. Only the EPAC2 isoform directly correlates with the ST2 gene level (Spearman r = 0.74, p < 0.0001). No differences were found between the EPAC1 and ST2 gene.

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