Figure 5

Ormeloxifene sensitizes Caski cells to radiation. Ormeloxifene radio-sensitizes cells and decreases cell proliferation. (A) Caski cells were pretreated with 10 µM ormeloxifene for 6 hours and next exposed to 4Gy radiation. Live cells were counted using a coulter counter. Error bars show SEM, n = 3. *p < 0.05. (B) Representative images of ormeloxifene and radiation treated cells. Images were taken at 100X. Ormeloxifene radio-sensitizes cells and decreases colony forming ability. (C) Caski cells were pretreated with 2, 4, 6 and 8 µM ormeloxifene for 6 hours and next exposed to 4Gy radiation. After 14 days, cells with combination treatment showed fewer colonies than compared to ormeloxifene and radiation alone. Error bars show SEM, n = 3. *p < 0.05. (D) Images represent inhibited clonogenic potential. Images were taken at 200X.