Figure 5
From: Dual resistance of transgenic plants against Cymbidium mosaic virus and Odontoglossum ringspot virus
Long-distance movement of CymMV and ORSV in WT and transgenic pH7W-COCP line 3 (pH) by grafting assay. (A) Flowchart of grafting. (B) Symptoms of grafting plants inoculated with C+O at 17 dpi. (C,D) RT-PCR analysis of CymMV and ORSV RNA accumulation in grafted stock leaves at 11 dpi (C) and systemic scion leaves at 17 dpi (D). CymMV was detected by primer pair (CymMV RdRp-F and CymMV RdRp-R) of 152 bp; ORSV by primer pair (ORSV-RdRpPai-F and ORSV-RdRpPai-R) of 860 bp and BaMV by primer pair (BaMV-P2-F and BaMV-P2-R) of 416 bp, respectively. Actin was an internal control.
