Figure 3 | Scientific Reports

Figure 3

From: Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control

Figure 3

Effect of NDs and ND-Neb-colloostatin on the morphology and viability of T. molitor hemocytes. The hemocytes 1 day after the topical application of: NDs at a dose of (B) 5 µg or (E) 41.5 µg or ND-Neb-colloostatin at a dose of (D) approximately 4.15 µg of NDs and 0.85 µg of Neb-colloostatin or (G) 41.5 µg of NDs and 8.5 µg of Neb-colloostatin per insect. Control beetles topically received (A) physiological solution or Neb-colloostatin at a dose of (C) 0.85 µg or (F) 8.5 µg of peptide per insect. All hemocytes were stained with SR-VAD-FMK reagent for caspase activity detection (red color), with Oregon Green phalloidin for F-actin cytoskeleton staining (green color) and with DAPI for DNA staining (blue color).

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