Figure 5

Cell type-specific nuclear size measurements in leaf tissue sections stained with propidium iodide (PI) at major tumor development stages. Data shows nuclear size measurements 4 dpi and 6 dpi in mock treated, SG200 infected and SG200Δsee1 infected PI stained leaf tissue sections. Analyzed cell types included Mesophyll and resulting hypertrophic tumor cells, as well as bundle sheath cells and resulting hyperplasic tumor cells. A minimum of 70 nuclei was measured per tissue type. Results represent the mean ± SD from three independent leaf sections per biological replicate. Two independent biological experiments were performed. Asterisks indicate statistical significance of nuclear size compared to mock treated tissue of the same age. P-values were calculated using the unpaired student’s t-test; ***p ≤ 0.001.