Table 1 Summary of samples used for BMAA analysis and SIA; n refers to the number of replicates.
From: Insufficient evidence for BMAA transfer in the pelagic and benthic food webs in the Baltic Sea
Source | Location | Date | Collection method and sample preparation | Organism size | BMAA (n) | SIA(n) |
|---|---|---|---|---|---|---|
Benthic food chain | ||||||
Sediment | Askö area (see Fig. S1) | 7 Jun 28 Jun 23 Sep | Upper 1–2 cm of sediment were collected with a benthic sled; the macrofauna were extracted with 1-mm sieve and used as benthic samples. The sediment was frozen in bulk at −20 °C | Not applicable | 6 | 5 |
Amphipod Monoporeia affinis | Sieved from the sediment, sorted by species and size class based on the visual inspection and frozen in bulk at −20 °C | 4.2 mg DW/ind. | 6 | 3 | ||
Baltic clam Macoma baltica | 9.3 mg DW/ind. | 6 | 5 | |||
Priapulid Halicryptus spp. | 15.8 mg DW/ind. | 4 | 6 | |||
Polychaete Marenzelleria spp. | 11.1 mg DW/ind. | 5 | 4 | |||
Flounder Platichthys flesus | Kvädö-fjärden | 12 Sep | Collected within SNMMP and stored at −30 °C in the Environmental Specimen Bank of the Swedish Natural History Museum | 21.3 cm | 3 | 5 |
Perch Perca fluviatilis | 19.2 cm | 3 | 3 | |||
Pelagic food chain | ||||||
Phytoplankton | Landsort Deep | 19 Jun 8 Aug | Tows were taken with a small plankton net (10 µm) in the upper 20 m. Crustacean zooplankton (Acartia spp., Eurytemora affinis, and Bosmina coregoni) were separated by a light trap, picked with forceps under a compound microscope, and frozen at −20 °C. The rest of the material was filtered (5 µm) and used as phytoplankton samples (>20 mg wet mass sample−1). As detritus and heterotrophic protists were present, these samples were also referred to as seston. | >10 µm | 5 | 2 |
Zooplankton | 19 Jun 8 Aug | 18.1 µg DW/ind. | 5 | 4 | ||
Mysid Mysis mixta | 12 Jun 15 Aug | Sampled with a WP2 (100 µm) plankton net in the upper 100 m, identified to species level and frozen individually in Eppendorf tubes at −20 °C. | 5.5 mg DW/ind. | 4 | 4 | |
Mysid Neomysis integer | 3.9 mg DW/ind. | 4 | 3 | |||
Herring Clupea harengus | 16 Sep | Collected within SNMMP and stored at −30 °C in the Environmental Specimen Bank of the Swedish Natural History Museum. | 16.7 cm | 3 | 5 | |
