Figure 4

Expression of ipaD under different temperature conditions as determined by qRT-PCR. Total RNA was harvested from HT073016, HT073016(virF+) and S. flexneri strain 301 cultivated at 25 °C and 37 °C. Relative transcript levels were calculated using the ∆∆CT method and fold changes in comparison to HT073016 at 37 °C. All values have been normalized to the endogenous reference gene recA. Means and standard deviations stand for three independent experiments were shown with * being p < 0.05 in the indicated comparisons. Error bars are +SD.