Figure 4 | Scientific Reports

Figure 4

From: Loss of Ku’s DNA end binding activity affects telomere length via destabilizing telomere-bound Est1 rather than altering TLC1 homeostasis

Figure 4

TLC1 levels are not stabilized in Ku mutant strains expressing CDC13-EST1 fusions. (A) Analysis of TLC1 levels by northern blotting of 1X-4X serial streakouts of cdc13Δ (WT), cdc13Δ yku70-R456E (yku70-R456E), and cdc13Δ yku70Δ (yku70Δ) strains expressing either Cdc13 or a Cdc13-Est1 fusion. Blots were also probed with ACT1 for loading. Full-length blots are presented in Supplementary Fig. S11. (B) Quantification of the mean from three independent northern blotting experiments. Error bars represent ± standard error of the mean (SEM).

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