Figure 4
Changes in α-CA gene expression in the posterior mantle edge. Expression of MgNACR and MgCA1 was analysed in the posterior region of the mantle edge in mussels that were exposed for two-days or two-weeks to lower water salinity (BW) or control full seawater group (SW). Gene expression levels were normalized using the geometric mean of two reference genes (ef1α and 18s). The results are represented as mean ± SEM of four to six biological replicates. Prism GraphPad v5 software was used to assess the significance of differences between the experimental groups using a Mann-Whitney (two-tailed) test (*p < 0.05). SWF: seawater fed, BWF: brackish water fed; SW: seawater fast; BW: brackish water fast.
