Figure 5
Protonogram and Coomassie blue SDS-PAGE of Mediterranean mussel (Mg) and Pacific oyster (Cg) mantle protein extracts. (A) Protonogram; (B) negative protonogram/absence of substrate and (C) SDS-PAGE stained with blue Coomassie of native crude protein extracts (≈ 1 mg/well) of the posterior mantle. The positive control (+) corresponded to commercial bovine CA II (BCA II, 5 μg/well). Samples were resolved on a 12% SDS-PAGE gel without reducing agent or thermal denaturation. The white arrows indicate the hydratase activity of the most abundant transcript MgNACR α-CA that has an estimated molecular weight of 43.3 kDa. In oyster protein extracts an intense reaction was observed for a protein of a similar size. M: molecular weight marker (PageRuler Plus, Thermo Scientific).
