Table 1 List of the primer sequences used in the study.

From: Evolution and diversity of alpha-carbonic anhydrases in the mantle of the Mediterranean mussel (Mytilus galloprovincialis)

Name

Sequence (5′-3′)

Annealing Temp. (°C)

Efficiency (%)

R2

18S Fwd

GTGCTAGGGATTGGGGCTTG

58

99.9

0.99

18S Rev

TAGTAACGACGGGCGGTGTG

   

Ef1alpha Fwd

GAAGGCTGAGCGTGAACGTG

58

100.4

0.99

Ef1alpha Rev

TCCTGGGGCATCAATAATGG

   

MgCA1 Fwd

CTCCATTGGTTGTCAAATATG

58

96.9

0.99

MgCA1 Rev

ATCGATTGTGTGTTCAGAAC

   

MgNACR Fwd

AGTGTCAGTGTCCTTCGTTGA

64

97.3

0.99

MgNACR Rev

TGCGCAGGTCGTCCAACAT

   
  1. The annealing temperature and the efficiency (%) of the primer pairs and the linearity R2 of the standard curve are indicated.
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