Figure 2 | Scientific Reports

Figure 2

From: DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing

Figure 2

Quality control parameters of fresh and preserved human cells. Distribution and median gene count per cell (a), unique molecular identifier (UMI) count per cell (b) and percentage of mitochondrial transcripts per cell (c) detected for fresh cells and after DMSO cryopreservation (DMSO), methanol fixation (MeOH) and storage in CellCover reagent at 4 °C (CC4) and −20 °C (CC20). Cells were stored for one (W01) and 15 weeks (W15). Data shown are derived from human HEK293 cells of the species mixing experiment.

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