Figure 3

Decreased ICAM-1 expression in pancreas of CVB3-infected Muc-1^KO mice compared to pancreas of infected WT mice. IHC staining of (A) NFκB p65 and (C) ICAM-1 on pancreas sections from WT and Muc-1^KO mice with/without CVB3 infection 8-days post-inoculation. Arrows point to the areas represented by the insert. NFκB p65 nuclear staining is observed in infiltrating immune cells and ductal like cells in WT mice that were infected with CVB3. Scale bar 60 μm. Quantification of nuclear-staining (B) NFκB p65 positive cells and (D) ICAM-1 expression levels collected from CVB3-infected Muc-1 WT and Muc-1^KO mice pancreas. **p ≤ 0.01. ***p ≤ 0.001. (E) Immunofluorescence co-staining of F4/80 (green), CVB3 (red), and ICAM-1 (magenta) in WT and Muc-1^KO mice with/without CVB3 infection 8 days post-inoculation. Blue staining indicates DAPI nuclear staining.