Figure 4
Lactate transporter expression. MCF-7 cells treated with HRG, lactate, and BPTES. The MCF-7 derived knockdown shRNA cells were pretreated with BPTES for 1 hr. The cells were then stimulated with HRG, lactate or vehicle for 48 hrs. The MCF-7 derived scrambled shRNA cells were pre-treated with BPTES for 1 hr. Muscle tissue extract was prepared from quadriceps femoris obtained from mouse. The cells were then stimulated with HRG, lactate or vehicle for 48 hrs. Cell extracts (0.1 mg) and muscle tissue extract (60 µg) were resolved by SDS-PAGE and Western blot analysis was performed with the indicated antibodies. Different areas of the nitrocellulose membrane are separated by white space. The space below the Western blots represents the measured band intensities normalized to actin.
