Figure 3

Two sequential hybridizations showing that (A) one copy of X chromosome has two peri-centromere regions (aqua) with missing Xpter (arrows), while the derivative X has interstitial Xpter signal (yellow; arrow head/inset), the FISH “cocktail” also contains probes for 1p (green) and 1q (red) and (B) re-hybridization confirming the derivative X has Xqter signal (yellow) on either end (arrows and arrow head in the inset), the FISH “cocktail” also containing probes for 2p (green) and 2q (red). (C) Translocation of PML locus (15q22; aqua) and the sub-telomere region (15qter; yellow) from chromosome 15 (green box; arrow heads/inset) to two copies of chromosome 1 (red circle; arrows/inset). The FISH “cocktail” also contains probes for the sub-telomere regions of chromosome 10 (red and green). (D) 15q21 locus is present on chromosome 15 (green box; arrows/inset), not on chromosome 1. (E) Confirmation of 1q31 (green) and 1q32 (red) regions are present on three copies of chromosome 1 (red circles; arrows/inset). (F) The same metaphase after two sequential hybridizations showing insertion of 19qter into another chromosome (red circle; arrow/inset); (G) re-hybridization of the same metaphase confirming the insertion is distal to 11pter as evident from sub-telomere hybridization of p (green) and q (red) arm of three copies chromosome 11 (red circles; arrows/inset); the FISH “cocktail” also contains probes for the sub-telomere (yellow) and centromere (aqua) regions of chromosome 18, and (H) absence of 3p21 on the derivative chromosome 11 (green box; arrow/inset), but present on the two copies of chromosome 3 (red circles; arrow heads/inset). (I) The same metaphase after two sequential hybridizations showing translocation of 9qter (red) from chromosomes 9 (red circles; arrow heads/inset) to a group D chromosome (arrow); the FISH cocktail also contains positive hybridization for sub-telomere (17qter; yellow) and centromere (aqua) regions of chromosome 17 and (J) re-hybridization of the same metaphase confirming the receptor chromosome is chromosome 13 as detected by 13qter (yellow) and 13q14 (aqua) and indicated by arrows in the inset; the FISH “cocktail” also contains probes for 6p (green) and 6q (orange).