Figure 6
2HG discriminates control from XL765-treated U87IDHmut tumor extracts. (A) A typical 500 MHz 1H NMR spectrum of the water fraction of tumor extract from a control animal. Numbers indicate the following metabolites: 1. Alanine, 2. N-acetylaspartate, 3. 2HG, 4. Glutamate, 5. Glutamine, 6. Aspartate, 7. Total creatine, 8. Total choline, 9. Myo-Inositol. *: speak (2.7 ppm) related to protease inhibitor solution, #: methanol residual. (B) Three-dimensional scatter plot of PCA shows discrimination of control (dark purple) and XL765-treated (light purple) groups. (C) Loading plot for 1H NMR. The correlation coefficient corresponding to OPLS-DA model is represented as color map. Numbers indicate the same metabolites as in (A). OPLS-DA: Orthogonal partial least squares discriminant analysis.
