Figure 7
Glucose and glutamine flux to 2HG is reduced in NHAIDHmut and U87IDHmut cells under XL765 treatment. (A,B) Representative 13C-spectra ([1-13C]glucose labeling) of NHAIDHmut and U87IDHmut control cells respectively. Inserts: Expansion of [4-13C]2HG and [4-13C]glutamate region for XL765-treated (top) and control (bottom). (C,D) Representative 13C-spectra ([3-13C]glutamine labeling) of NHAIDHmut and U87IDHmut control cells respectively. Inserts: Expansion of [3-13C]2HG and [3-13C]glutamate region for XL765 treatment (top) and control (bottom). (E–H) Average 2HG produced from [1-13C]glucose and [3-13C]glutamine (13C) and total 2HG levels (1H) for control and XL765-treated NHAIDHmut cells (E,F) and U87IDHmut cells (G,H). Dark blue/purple bar: Control; Light blue/purple bar: XL765-treated; Full bar: Steady state; Empty bar: Glucose derived metabolite; Hashed bar: Glutamine derived metabolite.
