Table 2 Quantitative comparison of metabolites quantified from the aqueous phase of control and XL765-treated U87IDHmut cell extract that presented correlation coefficient |r| higher than 0.8 in the S-plot (Fig. 3C).
Metabolite | Control group concentration (mean ± STD) [fmol/cell] | XL765 group concentration (mean ± STD) [fmol/cell] | p-value | Change under XL765 treatment [%] |
|---|---|---|---|---|
Alanine | 0.82 ± 0.34 | 0.06 ± 0.04 | <0.001 | −92.13 |
2HG | 1.49 ± 0.30 | 0.31 ± 0.09 | <0.001 | −79.38 |
Glutamate | 4.47 ± 0.90 | 1.79 ± 0.40 | <0.001 | −60.04 |
Glutamine | 5.26 ± 2.40 | 1.02 ± 0.63 | <0.001 | −80.56 |
Glutathione | 0.67 ± 0.22 | 0.48 ± 0.10 | 0.025 | −29.02 |
Aspartate | 13.93 ± 5.15 | 3.30 ± 0.48 | <0.001 | −76.3 |
Creatine | 0.67 ± 0.32 | 0.16 ± 0.06 | 0.001 | −75.63 |
Phosphocreatine | 0.41 ± 0.24 | 0.16 ± 0.06 | 0.060 | −60.58 |
Choline | 0.03 ± 0.04 | 0.16 ± 0.11 | 0.006 | 600.78 |
Phosphocholine | 2.20 ± 1.49 | 0.18 ± 0.13 | 0.008 | −70.99 |
Glycerylphosphorylcholine | 8.34 ± 2.27 | 0.64 ± 0.24 | <0.001 | −48.45 |
AXP | 4.44 ± 1.21 | 4.30 ± 1.22 | <0.001 | −57.02 |
Phenylalanine | 1.04 ± 0.38 | 0.64 ± 0.21 | 0.010 | −38.29 |
Formate | 1.09 ± 0.26 | 0.51 ± 0.17 | <0.001 | −52.91 |
NAD(P)H/+ | 0.52 ± 0.16 | 0.19 ± 0.03 | <0.001 | −64.77 |
NAD(P)+ | 0.24 ± 0.11 | 0.08 ± 0.03 | 0.001 | −64.66 |
